pro insulin staining Search Results


α sma proteintech  (Proteintech)
93
Proteintech α sma proteintech
FIGURE 3 | Kindlin-3 promotes angiogenesis in vivo. (A) Representative images of Masson's trichrome staining and quantitative data illustrat- ing the extent of fibrosis in heart sections from the indicated groups (n = 6). Scale bars = 500 μm. (B) Four weeks post-MI, ventricular tissues were analysed using immunofluorescence staining to evaluate capillary (CD31) and arteriolar <t>density</t> <t>(α-SMA)</t> in AAV-NC and AAV-K3 mice post-MI or Sham operation, including the remote area (RA) and border area (BA, n = 6). (C) Representative images and quantitative analysis of Western blots demonstrate the expression of angiogenesis-related proteins in mice 28 days post-MI (n = 3). Data were presented as mean ± SEM. ns, not significant, *p < 0.05, **p＜0.01.
α Sma Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/α sma proteintech/product/Proteintech
Average 93 stars, based on 1 article reviews
α sma proteintech - by Bioz Stars, 2026-05
93/100 stars
  Buy from Supplier
immunoreactive pro-insulin  (ScyTek Inc)
90
ScyTek Inc immunoreactive pro-insulin
FIGURE 3 | Kindlin-3 promotes angiogenesis in vivo. (A) Representative images of Masson's trichrome staining and quantitative data illustrat- ing the extent of fibrosis in heart sections from the indicated groups (n = 6). Scale bars = 500 μm. (B) Four weeks post-MI, ventricular tissues were analysed using immunofluorescence staining to evaluate capillary (CD31) and arteriolar <t>density</t> <t>(α-SMA)</t> in AAV-NC and AAV-K3 mice post-MI or Sham operation, including the remote area (RA) and border area (BA, n = 6). (C) Representative images and quantitative analysis of Western blots demonstrate the expression of angiogenesis-related proteins in mice 28 days post-MI (n = 3). Data were presented as mean ± SEM. ns, not significant, *p < 0.05, **p＜0.01.
Immunoreactive Pro Insulin, supplied by ScyTek Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/immunoreactive pro-insulin/product/ScyTek Inc
Average 90 stars, based on 1 article reviews
immunoreactive pro-insulin - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
pro insulin staining  (R&D Systems)
94
R&D Systems pro insulin staining
A) Pancreas sections from treated mice were stained for glucagon (green) and insulin (red) to visualize islets and assess their size. Blue: DAPI. Scale bars, 50 μm. B) Quantification of total islet size in sections from 22 islets from pancreas sections of 7 LFD beta-C3-KO mice, 33 islets from pancreas sections of 5 HFD beta-C3-KO mice, and 35 islets from pancreas sections of 6 HFD C3-flox mice. C) Fraction of total islet staining for insulin, from the same islets analysed in (B). D) Examples of staining of individual HFD islets for glucagon (green) <t>and</t> <t>pro-insulin</t> (red). E) Quantification of pro-insulin staining in islets from each group of mice. F) Quantification of pro-insulin in serum of mice at given time points, measured by pro-insulin specific ELISA. G) Transmission electron micrographs of pancreatic islet cells isolated from beta-C3-KO or C3-flox mice. Beta-C3-KO mouse beta-cells contain accumulated amounts of swollen ER or similar vesicular organelles (black arrows), while normal ER is apparent in C3-flox mouse islet cells (white arrows). See also . Increased amounts of autophagic material were also observed in beta-C3-KO cells (asterisk). H) In vitro insulin secretion from isolated islets from C3-flox or beta-C3-KO mice HFD-fed mice, at 15 or 60 min. I) Average insulin content values per islet from secretion experiment in panel (H) (average values shown for each of 6 mice: from 6 wells, 5 islets per well). J) Serum C3 levels in C3-flox or beta-C3-KO mice at the endpoint of HFD, as measured by ELISA. Statistics in E, one-way ANOVA, in F/H, two-way ANOVA, in I, Student’s T-test, and in B/C, by mixed model statistical analysis (see methods).
Pro Insulin Staining, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pro insulin staining/product/R&D Systems
Average 94 stars, based on 1 article reviews
pro insulin staining - by Bioz Stars, 2026-05
94/100 stars
  Buy from Supplier
mouse anti-human pro-insulin  (Merck KGaA)
90
Merck KGaA mouse anti-human pro-insulin
A) Pancreas sections from treated mice were stained for glucagon (green) and insulin (red) to visualize islets and assess their size. Blue: DAPI. Scale bars, 50 μm. B) Quantification of total islet size in sections from 22 islets from pancreas sections of 7 LFD beta-C3-KO mice, 33 islets from pancreas sections of 5 HFD beta-C3-KO mice, and 35 islets from pancreas sections of 6 HFD C3-flox mice. C) Fraction of total islet staining for insulin, from the same islets analysed in (B). D) Examples of staining of individual HFD islets for glucagon (green) <t>and</t> <t>pro-insulin</t> (red). E) Quantification of pro-insulin staining in islets from each group of mice. F) Quantification of pro-insulin in serum of mice at given time points, measured by pro-insulin specific ELISA. G) Transmission electron micrographs of pancreatic islet cells isolated from beta-C3-KO or C3-flox mice. Beta-C3-KO mouse beta-cells contain accumulated amounts of swollen ER or similar vesicular organelles (black arrows), while normal ER is apparent in C3-flox mouse islet cells (white arrows). See also . Increased amounts of autophagic material were also observed in beta-C3-KO cells (asterisk). H) In vitro insulin secretion from isolated islets from C3-flox or beta-C3-KO mice HFD-fed mice, at 15 or 60 min. I) Average insulin content values per islet from secretion experiment in panel (H) (average values shown for each of 6 mice: from 6 wells, 5 islets per well). J) Serum C3 levels in C3-flox or beta-C3-KO mice at the endpoint of HFD, as measured by ELISA. Statistics in E, one-way ANOVA, in F/H, two-way ANOVA, in I, Student’s T-test, and in B/C, by mixed model statistical analysis (see methods).
Mouse Anti Human Pro Insulin, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti-human pro-insulin/product/Merck KGaA
Average 90 stars, based on 1 article reviews
mouse anti-human pro-insulin - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
recombinant mouse insulin-like growth factor binding protein 3  (Creative BioMart)
N/A
Recombinant Mouse Igfbp3 was fused to the human CD33 signal peptide. This chimeric protein was expressed inNSO cells.Insulin-like growth factor binding protein 3, also known as IGFBP3, is a member of the insulin-like growth factor
   Buy from Supplier
recombinant human insulin protein  (Novus Biologicals)
N/A
The Recombinant Human Insulin Protein has been validated for the following applications Western Blot ELISA Protein Array Immunoaffinity Purification
   Buy from Supplier
recombinant mouse insulin r cd220 protein cf  (R&D Systems)
N/A
The Recombinant Mouse Insulin R CD220 Protein from R D Systems is derived from CHO The Recombinant Mouse Insulin R CD220 Protein has been validated for the following applications Bioactivity
   Buy from Supplier
recombinant mouse insulin-like growth factor binding protein 6  (Creative BioMart)
N/A
Insulin-like growth factor binding protein 6, also known as IGFBP6 an extracellular protein with preferential affinity for insulin-like growth factor (IGF) II. IGFBP6 plays a role in lipoprotein assembly and dietary cholesterol absorption. In addition
   Buy from Supplier
recombinant human insulin r cd220 aa 28 944 protein cf  (R&D Systems)
N/A
The Recombinant Human Insulin R CD220 aa 28 944 Protein from R D Systems is derived from NS0 The Recombinant Human Insulin R CD220 aa 28 944 Protein has been validated for the following applications
   Buy from Supplier
recombinant rat insulysin ide protein cf  (R&D Systems)
N/A
The Recombinant Rat Insulysin IDE Protein from R D Systems is derived from Sf 21 baculovirus The Recombinant Rat Insulysin IDE Protein has been validated for the following applications Enzyme Activity
   Buy from Supplier
recombinant mouse insulin-like growth factor binding protein 1  (Creative BioMart)
N/A
RecombinantMouse Igfbp1 protein was expressed in Murine myeloma cellline. Ala26-Asn272.Theinsulin-like growth factor binding protein (IGFBP) family consists of six structurallyrelated proteins that bind IGF with high affinity. These proteins share conservedcysteine-rich N- and C-terminal regions
   Buy from Supplier
recombinant mouse insulin-like growth factor binding protein 5  (Creative BioMart)
N/A
RecombinantMouse Igfbp5 protein was expressed in Murine myeloma cellline. Val15-Glu271.Thesuperfamily of insulin-like growth factor (IGF) binding proteins include thesix high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinitybinding proteins referred to as IGFBP
   Buy from Supplier

Image Search Results


FIGURE 3 | Kindlin-3 promotes angiogenesis in vivo. (A) Representative images of Masson's trichrome staining and quantitative data illustrat- ing the extent of fibrosis in heart sections from the indicated groups (n = 6). Scale bars = 500 μm. (B) Four weeks post-MI, ventricular tissues were analysed using immunofluorescence staining to evaluate capillary (CD31) and arteriolar density (α-SMA) in AAV-NC and AAV-K3 mice post-MI or Sham operation, including the remote area (RA) and border area (BA, n = 6). (C) Representative images and quantitative analysis of Western blots demonstrate the expression of angiogenesis-related proteins in mice 28 days post-MI (n = 3). Data were presented as mean ± SEM. ns, not significant, *p < 0.05, **p＜0.01.

Journal: Journal of cellular and molecular medicine

Article Title: Kindlin-3 Promotes Angiogenesis via Notch Signalling and Is Crucial for Functional Recovery Postmyocardial Infarction.

doi: 10.1111/jcmm.70494

Figure Lengend Snippet: FIGURE 3 | Kindlin-3 promotes angiogenesis in vivo. (A) Representative images of Masson's trichrome staining and quantitative data illustrat- ing the extent of fibrosis in heart sections from the indicated groups (n = 6). Scale bars = 500 μm. (B) Four weeks post-MI, ventricular tissues were analysed using immunofluorescence staining to evaluate capillary (CD31) and arteriolar density (α-SMA) in AAV-NC and AAV-K3 mice post-MI or Sham operation, including the remote area (RA) and border area (BA, n = 6). (C) Representative images and quantitative analysis of Western blots demonstrate the expression of angiogenesis-related proteins in mice 28 days post-MI (n = 3). Data were presented as mean ± SEM. ns, not significant, *p < 0.05, **p＜0.01.

Article Snippet: Name of antibody Manufacturer Identifier Concentration Kindlin- 3 Affinity Biosciences DF13558 1:1000 (WB), 1:200 (IHC) VEGF Proteintech 19003- 1- AP 1:1000 (WB) β1- integrin Bioss bs- 0486R 1:2000 (WB), 1:400 (IHC) GAPDH Proteintech 6004- 1- Ig 1:2000 (WB) Hif- 1α Affinity Biosciences BF8002 1:2000 (WB) eNOS Proteintech 27120- 1- AP 1:1000 (WB) p- eNOS Proteintech 28937- 1- AP 1:1000 (WB) CD31 Proteintech 11265- 1- AP 1:1000 (WB), 1:400 (IF) Cleaved Caspase 3 Cell Signaling Technology 9664 1:1000 (WB) Caspase 3 Cell Signaling Technology 9662 1:1000 (WB) α- Actinin Sigma A7811 1:400 (IF) α- SMA Proteintech 14395- 1- AP 1:400 (IF) vWF Proteintech 66682- 1- Ig 1:400 (IF) Notch1 Proteintech 20687- 1- AP 1:1000 (WB), 1:400 (IHC) Hes1 Affinity Biosciences DF7569 1:1000 (WB) HRP- conjugated a Goat Anti- Rabbit Servicebio GB233303 1:200 (IHC) Goat Anti- Rabbit IgG, Peroxidase Conjugated, H + L Biosharp BL003A 1:10000 (WB) Goat Anti- Mouse IgG, Peroxidase Conjugated, H + L Biosharp BL001A 1:10000 (WB) Alexa Fluor 488- conjugated AffiniPure Goat AntiRabbit IgG (H + L) Jackson 115- 585- 003 1:200 (IF) Alexa Fluor 594- conjugated AffiniPure Goat AntiMouse IgG (H + L) Jackson 115- 585- 003 1:200 (IF) Abbreviations: IF, immunofluorescence; IHC, immunohistochemistry; WB, Western blot. nloaded from https://onlinelibrary.w iley.com /doi/10.1111/jcm m .70494 by IN A SP - N E PA L , W iley O nline L ibrary on [21/03/2025].

Techniques: In Vivo, Staining, Immunofluorescence, Western Blot, Expressing

A) Pancreas sections from treated mice were stained for glucagon (green) and insulin (red) to visualize islets and assess their size. Blue: DAPI. Scale bars, 50 μm. B) Quantification of total islet size in sections from 22 islets from pancreas sections of 7 LFD beta-C3-KO mice, 33 islets from pancreas sections of 5 HFD beta-C3-KO mice, and 35 islets from pancreas sections of 6 HFD C3-flox mice. C) Fraction of total islet staining for insulin, from the same islets analysed in (B). D) Examples of staining of individual HFD islets for glucagon (green) and pro-insulin (red). E) Quantification of pro-insulin staining in islets from each group of mice. F) Quantification of pro-insulin in serum of mice at given time points, measured by pro-insulin specific ELISA. G) Transmission electron micrographs of pancreatic islet cells isolated from beta-C3-KO or C3-flox mice. Beta-C3-KO mouse beta-cells contain accumulated amounts of swollen ER or similar vesicular organelles (black arrows), while normal ER is apparent in C3-flox mouse islet cells (white arrows). See also . Increased amounts of autophagic material were also observed in beta-C3-KO cells (asterisk). H) In vitro insulin secretion from isolated islets from C3-flox or beta-C3-KO mice HFD-fed mice, at 15 or 60 min. I) Average insulin content values per islet from secretion experiment in panel (H) (average values shown for each of 6 mice: from 6 wells, 5 islets per well). J) Serum C3 levels in C3-flox or beta-C3-KO mice at the endpoint of HFD, as measured by ELISA. Statistics in E, one-way ANOVA, in F/H, two-way ANOVA, in I, Student’s T-test, and in B/C, by mixed model statistical analysis (see methods).

Journal: Molecular Metabolism

Article Title: Beta-cell-specific C3 deficiency exacerbates metabolic dysregulation and insulin resistance in obesity

doi: 10.1016/j.molmet.2025.102302

Figure Lengend Snippet: A) Pancreas sections from treated mice were stained for glucagon (green) and insulin (red) to visualize islets and assess their size. Blue: DAPI. Scale bars, 50 μm. B) Quantification of total islet size in sections from 22 islets from pancreas sections of 7 LFD beta-C3-KO mice, 33 islets from pancreas sections of 5 HFD beta-C3-KO mice, and 35 islets from pancreas sections of 6 HFD C3-flox mice. C) Fraction of total islet staining for insulin, from the same islets analysed in (B). D) Examples of staining of individual HFD islets for glucagon (green) and pro-insulin (red). E) Quantification of pro-insulin staining in islets from each group of mice. F) Quantification of pro-insulin in serum of mice at given time points, measured by pro-insulin specific ELISA. G) Transmission electron micrographs of pancreatic islet cells isolated from beta-C3-KO or C3-flox mice. Beta-C3-KO mouse beta-cells contain accumulated amounts of swollen ER or similar vesicular organelles (black arrows), while normal ER is apparent in C3-flox mouse islet cells (white arrows). See also . Increased amounts of autophagic material were also observed in beta-C3-KO cells (asterisk). H) In vitro insulin secretion from isolated islets from C3-flox or beta-C3-KO mice HFD-fed mice, at 15 or 60 min. I) Average insulin content values per islet from secretion experiment in panel (H) (average values shown for each of 6 mice: from 6 wells, 5 islets per well). J) Serum C3 levels in C3-flox or beta-C3-KO mice at the endpoint of HFD, as measured by ELISA. Statistics in E, one-way ANOVA, in F/H, two-way ANOVA, in I, Student’s T-test, and in B/C, by mixed model statistical analysis (see methods).

Article Snippet: For pro-insulin staining, AlexaFluor647-labeled anti-pro-insulin (R&D Systems, #IC13361R, 1:100) was incubated overnight at 4 °C, in combination with glucagon staining, before washing and mounting as described above.

Techniques: Staining, Enzyme-linked Immunosorbent Assay, Transmission Assay, Isolation, In Vitro